Figure 1. Time-dependent results for semi-continuous batch cultures of Karenia brevis strain CCMP 2228 grown at nutrient-replete rates in high-P media (open squares) and grown into P-limitation in low-P media (filled circles).

(A) Biovolume (µL_cells/L_media) plotted on a log scale vs. time, (B) Natural logarithm (ln) biovolume vs. time, correcting for culture dilution [39], (C) Cellular phosphorus to carbon molar ratios (mmol/mol), (D) Mean volume per cell (µm³/cell), (E) Total brevetoxins per cell (pg/cell), (F) Chlorophyll a normalized to cell volume (fg/µm³), (G) Total brevetoxins normalized to cell volume (fg/µm³), (H) Percent of cellular carbon associated with brevetoxins (%C-PbTx). Error bars represent the standard deviation of triplicate measurements, except those normalized to carbon, where the cellular C was measured only in duplicate.