Figure 1. Purification of human granulocytes and precursors.
A, Cytospins (1–2×105 cells) of the MB/PM, MC/MM, BC/SC, and PMNs populations from one representative donor before and after immunomagnetic depletion of non-neutrophil cells. B, Real-time PCR data on RNA purified from the four populations in three donors. The expression of the marker mRNAs is shown relative to the cell population with the highest amount, which is given the value 1. MPO expression peak in the MB/PM population, Lactoferrin in the MC/MM population, and high expression of the fMLP-receptor is seen in the BC/SC and PMN populations. Error bars (standard deviation, SD) show the difference in expression between the three donors.