Table 1. Predicted targets for selected differentially regulated miRNAs in granulopoiesis.

miRNA	Predicted Targets
hsa-miR-130a	Both Targetscan and miRanda : MYB, SMAD4, CSF1, TGFBR2, MYBL1, CBFB, PTEN, HOXA5, IGF1, CREB1
	Targetscan: ACVR1, MLL3, CDK19, SMAD5, MAP3K12, TGFBR1, MAPK1, STAT3, E2F2.
	miRanda: IL7, TNF, IL22, IL15, CYBB, E2F7, STAT3, SMAD5, TLR7, MLL4, IL16, TGFBR3, MAP2K5, MAPK6, SMAD7, TAB1, E2F8, MEIS1, AKT3, MAP3K14, MLL5, IL26.
hsa-miR-155	Both Targetscan and miRanda: MYB, CEBPB, SP1, MAP3K14, MYBL1, SPI1, CUX1, E2F2, MEIS1, SP3, TAB2
	Targetscan: SMAD2, MAP3K10, TAB2, TRAF3, TBRG1,MYD88
	miRanda: FOS, MLL5, E2F5, E2F3, SP8, SMAD1, IL7, TGFA, OLFM4, GFI1, SMAD6, ELF1, EGFR, SMAD5, E2F2, E2F7, CDK7, MAP3K8, HOXA3, DEFA5, IL23R, JAK2, GATA3, CBFB, AKT2, IL13, TGFBR2, SP2, CYBB, CDK2, PTEN, CEBPD, STAT1, IL18R1, MAPK1, IL12A.
hsa-miR-146a	Both Targetscan and miRanda: TRAF6, IRAK1, SMAD4, MYBL1, NOTCH2, SP8.
	Targetscan: CUX1
	miRanda: TLR2, RUNX1T1, MLL5, TAB2, STAT1, CDK9, TGFBR1, RARA,TLR4, CDK1, RUNX1, IL6R, SP3, IL23R, FAS, MEIS1, IL3.
hsa-miR-34c-3p	Both Targetscan and miRanda: MAP3K2, FLT3, RUNX1T1.
	Targetscan: CDK17, PDGFB.
	miRanda: HOXA13, IL23R, CREB1, AKT3, HOXA2, IL1A, MAP4K5, FLT1, CYBB, TRAF5, CDK7, MMP8, GATA3, FOXP2, VCAM1, GFI1, ACTR3, IRAK4, CD34, MAP3K9, OLFM4, SP3, IL3RA, TAB2, PTPN9, IL15, IL19, CDK13, NOTCH1, CBFB, CSF1, CUX1, SMAD4, E2F2, F2F5, E2F7.
hsa-miR-99b	Both Targetscan and miRanda: MTOR, HOXA1.
	Targetscan: NOX4, IGFR1.
	MicroRNA.org: CYBB, MLL3, FAS, PIK3R3, CDK7.
hsa-miR-183	Both Targetscan and miRanda: PTPN4, CUX1.
	Targetscan: TGB1, FOXP1, FOX01, TAB3, MAP3K4, MAP3K13.
	miRanda: SMAD4, PIK3R1, MAPK9, MMP9, SMAD2, NFKB1, TLR8, TAB2, SP7, IL12B, E2F3, EGFR, FAS, CUX2, VCAM1, MTOR, RUNX1, E2F5, MAP2K6, NOTCH2, MYC, PTPN9, MAP3K5, MAPK7, IL8, HOXA5, IL23R, AKT1, MAP2K5, TRAF2, CDK4.
hsa-miR-26a	Both Targetscan and miRanda: CBFB, SP3, MYBL1, SP1, CUX1,
	Targetscan: CDC6, CDK8, TAB3, RB1, CREBBP.
	miRanda: IL8R1, MAP3K1, CDK8, TAB2, MAPK6, MAP3K8, MLL3, CREB1, JAK2, MAPK10, VEGFA, CDK12, SMAD6, MAPK13, TGFBR2, STAT2, MAP3K2, IL6, CYBB, HOXD8, SP8, MEIS2, HOXD13, HOXA4, CBFB, TRAF3, SMAD4, CDK1, SMAD5, TGFB2, E2F5, CDK14.
hsa-miR-223	Both Targetscan and miRanda: CBFB, SP3, MYBL1, SP1, CUX1,
	Targetscan: TGFBR3, CDK17, E2F1.
	miRanda: EGFR, STAT1, OLFM4, IL3, SMAD1, CDK9, MEIS1, ICAM1, MYC, GFI1, FAS, MAPK4, CDK8, NOTCH2, PTEN, IL23A, IL12B, NOTCH1.
hsa-miR-27a	Both Targetscan and miRanda: FOXP2, E2F7, CBFB, RUNX1, RARA, HOXA10, EGFR, TAB3.
	Targetscan: MAP2K4, HOXA5, HOXB8, HOXA13, SMAD9, CREB1, PKIA, TRAF3, SMAD5, CDK18, ITGA2, MLL3, FOXP4, MAPK10, SP6, CDK8, MAPK12.
	miRanda: FAS, MLL3, HOXB5, HOXA9, SMAD9, MAPK13, FOXA3, E2F5, MAPK9, SMAD1, MYB, IRAK4, IL23R, IL16, CSF1, CYBB, PTPN9, IL24, IL12A, MMP7, CDK12, CEBPE, MAP2, CDK18, MLL5, MLL, CEBPG, SMAD4, ICAM2, NOTCH1, PTEN, IL19, E4F1, STAT1.

The table lists the different predicted targets from two different prediction algorithms, miRanda and Targetscan. The targets are listed as predicted by both prediction algorithms or by either one and were selected based on their importance in granulopoiesis and granulocyte function.