Figure 2. Prox1 did not increase β-galactosidase staining in HCC cells with p53 deficiency. p53-deleted Hep3B cells or p53-mutated Mahlavu cells were transfected with pcDNA (Con) or Prox1 expression vector. After 72 h, cells were subjected to β-galactosidase staining and Prox1 protein level was studied by western blotting.