Figure 6. Prox1 inhibits tumor growth in vivo. (A) SK-HEP-1 cells were transfected with pcDNA or Prox1 expression vector and subjected to subcutaneous injection as described in Materials and Methods. Tumor growth was continuously monitored and mice were sacrificed at 14 d after injection. Tumors were removed and weighted. *p < 0.05 when compared with the control group. (B) Immunohistochemical staining of tumor tissues (upper panel) and immunocytochemical staining of control and Prox1- expressing SK-HEP-1 cells (bottom panel) were conducted. Representative images were shown. (C) Tumor tissue sections were stained with Ki-67, Twist and p53 antibodies and representative images were shown.