Figure 4. Lysis of HLA-A2-positive A673 cells by LIPI-stimulated CTL. T-cells from two HLA-A2-positive donors were stimulated with DC that were pulsed with SK-N-MC lysate, A673 lysate, CCND1 peptides RLT and LLG or LIPI peptides SLS, NLL and LDY. Cells were re-stimulated with HLA-A2-positive LCL and the same antigens. CTL that were primed with un-pulsed DC and re-stimulated with un-pulsed LCL served as negative controls. The cell mediated cytotoxicity of the CTL against living A673 tumor cells was analyzed in LDH assay.