Figure 1. RNAi-mediated stable knockdown of CDH17 in MKN-45 cells. (A) The plasmids of pRI-CMV/eGFP-CDH17-miR-1, -2, -3 and -neg detected by DNA sequencing were consistent with the designed miRNA insert fragments. (B) High expression of GFP was observed in lentiviral vector transfected cells, as opposed to the negative expression of it in MKN-45 cells receiving no treatment (100μm). (C) 94–98% of transfected cells are GFP positive, determined by flow cytometry analysis. (D) Real time RT-PCR indicated that the miR-CDH17(2) was the optimal sequence, with 80% interference efficacy of CDH17, which was confirmed by (E) western blot analysis.