Characteristics of repair of renal tubular cells in models of AKI. (A) Number (per 400× field) of Ki-67–positive (left), bromodeoxyuridine (BrdU)-positive (middle), or p-H3–positive (right) tubular cells in moderate IRI, unilateral IRI, and aristolochic acid nephropathy (AAN) mice (n = 3 mice at each time point in each group). *P < .01 versus sham. #
P < .01 versus control. Those mice in the moderate IRI and AAN groups went on to develop severe fibrosis over 42 days after the initial ischemia or dose of AA. (B) Cell-cycle distribution (G1, S, and G2/M) of tubular cells that have entered the cell cycle (Ki67+) in moderate IRI (left), unilateral IRI (middle), and AAN (right) models as a function of time after the insult (n = 3 mice at each time point in each group). Reprinted with permission from Yang et al.80