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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Cytokine. 2013 Jan 27;61(3):924–932. doi: 10.1016/j.cyto.2012.12.015

Fig. 4.

Fig. 4

Chemotaxis of L1.2.fCCR6 cells toward fCCL20. (A) Chemotaxis of L1.2.fCCR6 cells was performed toward the indicated concentration of synthetic fCCL20. The data are presented as the chemotactic index relative to migration toward no chemokine and are combined from two independent experiments, each performed in triplicate. (B) Chemotaxis of L1.2.fCCR6 cells and L1.2 parental cells was performed toward synthetic fCCL20 (100nM), or toward supernatants from HEK293T cells transiently expressing fCCL20-mIgG2a or fCXCL10-mIgG2a fusion proteins. The supernatants were used neat or at 1:10 or 1:100 dilutions, indicated by the concentration gradient wedges. The data are presented as the percent chemotaxis relative to the synthetic fCCL20 control, and are combined from two independent experiments both performed in triplicate.