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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Cell Signal. 2013 Jan 16;25(4):961–969. doi: 10.1016/j.cellsig.2013.01.007

Figure 4. ALDEFLUOR assay, Oct-4 immunofluorescent staining, and Oct-4 gene expression in mammosphere of non-stem cells.

Figure 4

Figure 4

(A) MDA-MB-231 cells from non-stem monolayer culture or duplicate mammosphere cultures (30 days) were labeled with the Aldefluor (BAAA) with and without the ALDH inhibitor DEAB and analyzed with flow cytometry. The numbers shown in each panel reflect the percentage of ALDH+ cells in each corresponding group. (B) Mammospheres from MDA-MB-231 and MDA-MB-453 stem-like cells or non-stem cells were cultured for 30 days (left panels), harvested by cytospin, and stained with DAPI or anti-Oct-4 antibody, and then displayed in overlay images.