Figure 5. Comparison of cytokine production in MDA-MB-231 stem-like cells and non-stem cells.
(A) Cells were plated in 60-mm Petri dishes or ultra-low attachment plates. Conditioned media from monolayer culture or mammosphere culture were harvested and subjected to cytokine antibody arrays. (B) The cytokine array image was analyzed with densitometer and average of area integration of optical intensities for each pair of cytokine spots was plotted.