Figure 3. Presence of EGFP⁺ CD4 T cells in IL-2^cre: Z/EG mice.

(A) Expression of EGFP by CD3⁺CD4⁺ cells in spleens from Z/EG control mice and IL-2^cre: Z/EG mice. The numbers indicate the percent of cells within each gated region. (B) Expression of EGFP by different subset of lymphocytes from spleen of IL-2^cre: Z/EG were analyzed by surface antigen staining for CD4, CD8, TCR, and NK1.1 as indicated. (C) Expression on IL-2 by EGFP^- and EGFP⁺ CD4 T cells. Splenic CD4 T cells from IL-2^cre: Z/EG were stimulated ex vivo by PMA and ionomycin for 4 hours. Expression of IL-2 was determined by intracellular staining and flow cytometry. (D) Expression of EGFP by CD3⁺CD4⁺ cells isolated from mesenteric lymph nodes (mLN), Peyer's patches (PP), intraepithelial lymphocyte of small intestine (IEL), and lamina propria of small intestine (LP), lung and bone marrow (BM) of IL-2^cre: Z/EG mice. The numbers indicate the percent of cells within each gated region. (E) Expression of EGFP by CD4⁺ single positive thymocytes isolated from Z/EG control mice and IL-2^cre: Z/EG mice. CD4⁺CD8^- cells were gated by analysis software from total thymocytes and analyzed for EGFP expression. (F) Expression of EGFP by non-CD4⁺ CD8^- thymocytes as analyzed in (E).