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. Author manuscript; available in PMC: 2014 Mar 7.
Published in final edited form as: Mol Cell. 2013 Jan 31;49(5):1010–1015. doi: 10.1016/j.molcel.2012.12.021

Figure 2. S-phase Checkpoint Activation is Eliminated by TOP1 Deletion.

Figure 2

Western blot detection of Rnr3 from whole cell extracts. Immunoblotting was performed using an antibody to Rnr3 or actin (loading control). Elevation of Rnr3 protein level is an indicator of S-phase checkpoint activation (Kumar et al., 2010). All strains harbor the pol2-M644G mutator allele.