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. Author manuscript; available in PMC: 2014 Mar 8.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Feb 10;432(2):226–230. doi: 10.1016/j.bbrc.2013.02.010

Figure 2. CCCP induces LC3 lipidation in cells with compromised Beclin 1/Atg14 complex.

Figure 2

(A–B). Wild type (wt) U251 cells and U251 cells with constitutive Beclin 1 knockdown (kd) were infected with Ad-GFP-LC3 overnight and then treated with CCCP or EBSS in the presence or absence of CQ. Cell lysates were analyzed by immunoblotting assays with indicated antibodies (A). Fluorescent images were taken for quantification of GFP-LC3 punctation (B). (C–D). HeLa cells expressing GFP-LC3 were transfected with Atg14-specific siRNAs for 48 hours and then treated with CCCP or EBSS in the presence or absence of CQ. Cell lysates were analyzed by immunoblotting assay with indicated antibodies (C). Fluorescent images were taken for quantification of GFP-LC3 punctation (D). *: P<0.05; **: P<0.01; ND: no statistical difference.