Table 1. Gene expression changes in HRPE cells treated with TGF-β.
HRPE cultures were grown to confluence were treated with TGF-β1(10 ng/ml) for 8h in serum free medium. Total RNA prepared was used for microarray analysis using Affymetrix GeneChip as described in Materials and Methods section.
| Affymetrix ID | Fold Change | Gene name |
|---|---|---|
| 215646_s_at | 17.83 | chondroitin sulfate proteoglycan 2 (versican) |
| 202310_s_at | 15.6 | collagen, type I, alpha 1 |
| 204619_s_at | 14.98 | chondroitin sulfate proteoglycan 2 (versican) |
| 202311_s_at | 14.54 | collagen, type I, alpha 1 |
| 213640_s_at | 14.51 | lysyl oxidase |
| 214701_s_at | 13.42 | fibronectin 1 |
| 201107_s_at | 13.07 | thrombospondin 1 |
| 205828_at | 12.6 | matrix metalloproteinase 3 (stromelysin 1, progelatinase) |
| 226535_at | 12.27 | integrin, beta 6 |
| 221731_x_at | 11.68 | chondroitin sulfate proteoglycan 2 (versican) |
| 226847_at | 11.54 | follistatin |
| 204298_s_at | 10.46 | lysyl oxidase |
| 204620_s_at | 10.32 | chondroitin sulfate proteoglycan 2 (versican) |
| 211571_s_at | 9.588 | chondroitin sulfate proteoglycan 2 (versican) |
| 201645_at | 9.249 | tenascin C (hexabrachion) |
| 215446_s_at | 8.532 | Human lysyl oxidase (LOX) gene, exon 7. |
| 204475_at | 7.224 | matrix metalloproteinase 1 (interstitial collagenase) |
| 214602_at | 6.707 | collagen, type IV, alpha 4 |
| 212865_s_at | 6.676 | collagen, type XIV, alpha 1 (undulin) |
| 214702_at | 6.637 | fibronectin 1 |
| 201109_s_at | 6.37 | thrombospondin 1 |
| 201108_s_at | 5.155 | thrombospondin 1 |
| 216893_s_at | 4.939 | collagen, type IV, alpha 3 (Goodpasture antigen) |
| 221729_at | 4.462 | collagen, type V, alpha 2 |
| 208083_s_at | 4.368 | integrin, beta 6 |
| 222073_at | 4.327 | collagen, type IV, alpha 3 (Goodpasture antigen) |
| 211980_at | 4.317 | collagen, type IV, alpha 1 |
| 211981_at | 3.998 | collagen, type IV, alpha 1 |
| 214641_at | 3.903 | collagen, type IV, alpha 3 (Goodpasture antigen) |
| 216898_s_at | 3.778 | collagen, type IV, alpha 3 (Goodpasture antigen) |
| 219697_at | 3.506 | heparan sulfate (glucosamine) 3-O-sulfotransferase 2 |
| 205206_at | 3.461 | Kallmann syndrome 1 sequence |
| 202620_s_at | 3.087 | procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase) 2 |
| 201069_at | 2.845 | matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) |
| 211966_at | 2.299 | collagen, type IV, alpha 2 |
| 212464_s_at | 2.199 | fibronectin 1 |
| 201656_at | 0.485 | integrin, alpha 6 |
| 219909_at | 0.481 | matrix metalloproteinase 28 |
| 239273_s_at | 0.302 | matrix metalloproteinase 28 |
| 239272_at | 0.221 | matrix metalloproteinase 28 |