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. Author manuscript; available in PMC: 2014 Apr 3.
Published in final edited form as: Neuroscience. 2013 Jan 18;235:174–186. doi: 10.1016/j.neuroscience.2012.12.069

Figure 2.

Figure 2

Exposure to intermittent alcohol vapor during adolescence increased Avp mRNA in the PVN of male rats on PND 42 but not PND 36. Male rats were exposed to alcohol vapor for 6 h per day from PND 28 and brains collected for analysis at the conclusion of the 6 h vapor exposure period on PND 36 and PND 42. (Left panel) Avp mRNA levels were quantified using optical density (arbitrary units) and normalized to average values of age-matched controls. Data are expressed as mean ± SEM percent control of 4–6 male rats per group. **, P<0.01. (Right panel) Representative dark-field photomicrographs (100X magnification) of PVN Avp mRNA in alcohol vapor- (EtOH) and air-exposed (CON) rats euthanized on PND 36 and PND 42.