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. Author manuscript; available in PMC: 2014 Apr 3.
Published in final edited form as: Neuroscience. 2013 Jan 18;235:174–186. doi: 10.1016/j.neuroscience.2012.12.069

Figure 4.

Figure 4

Exposure to intermittent alcohol vapor during adolescence increased the number of colocalized c-fos and PNMT immunoreactive (ir) cells/section and the number of PNMT-ir cells/section in the C2 region of the brain stem of male rats following adult alcohol challenge. Male rats exposed in adolescence to intermittent ethanol (AIE) or air controls (CON) were euthanized 2 h after administration of a 4.5 g/kg ig alcohol challenge (EtOH), or equivalent volume of water (VEH). (A) Double immunohistochemical staining labeled c-fos-containing nuclei black and PNMT-containing cell bodies brown. Representative images were captured using a 20X dry objective (scale bar=100 μm). (B) Quantification of PNMT-positive cell numbers (left) and PNMT-positive cells containing c-fos staining (right) are expressed as mean ± SEM cells/section for 4–6 male rats per treatment. ** P=0.01, a P=0.053.