Figure 2.

Using chemical probes to capture H3K9Me₃-binding proteins. (a) The concentration-dependent labeling of purified chromodomain of HP1 (GST-HP1_CD) by probe 2 (n = 2). (b) The recombinant GST-HP1_CD was selectively labeled by methylated probe 2 (3 μM) but not unmodified probe 2C (3 μM) and the labeling by 2 was inhibited by H3K9Me₃ peptide (30 μM). (c) Recombinant GST-HP1_CD and GFP-tagged endogenous Swi6, the human HP1 homolog, were selectively captured by probe 2 from fission yeast cell lysates. Cdc4 was the loading control.