Figure 1.

The characterization of the p239 particles. The p239 particles in PBS were analyzed using EM and determined to have a diameter of approximately 25 nm (A, bar = 100 nm). AUC demonstrated a sedimentation coefficient of approximately 23 S (B), and the GFC results revealed an elution time of 13.7 min (kav = 0.1980) (C). In addition, the DLS analysis showed a hydrated radius of approximately 13 nm (D), which is consistent with the EM data. The structural organization of HEV ORF2 and its different structural subunits containing cell-expressed rHEV and E. coli-expressed p239 and E2 were described (E).