Figure 2.
The size distribution of refolded p239 and E2 were investigated using AUC. SV (A) and SE (B) experiments show that the unfolded p239 existed as a dimer in urea. SV (C) and SE (D) experiments show that the unfolded E2 existed as a dimer in urea. The refolding of p239 (E) resulted in differences between the nontreated p239 [no (NH4)2SO4] and the (NH4)2SO4-treated p239 (the concentrations are labeled). The refolding of E2 (F) was similar between the nontreated E2 [no (NH4)2SO4] and the (NH4)2SO4-treated E2 (the concentrations are labeled). [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com.]