Figure 4.

Time-scale sedimentation coefficient distribution analysis of p239 with treatments of (NH₄)₂SO₄. The components of the p239 treatments are shown in histogram form (A), and the two column types representing the components at 25°C and 37°C are labeled. The concentration of (NH₄)₂SO₄ is also labeled. The c(s) distribution of the different species that were present following the 25°C incubation is shown in a line and symbol form (B). The sedimentation coefficient distributions of p239 and E2 following different (NH₄)₂SO₄ treatments are shown in a box chart (C). *, assembly intermediates with sedimentation coefficient around 5 S.