Fig. 4.

Effect of repeated exposure to chemotherapy on the expression of β-tubulin isotypes in endothelial cells. a Representative immunoblots of total endothelial cell lysates following long-term treatment with chemotherapy. Membranes were probed with antibodies directed against GADPH (loading control), βI-, βII-, βIII-, βIV- and total β-tubulin. b Histogram showing the relative expression of tubulin isotypes in the 6 BMH29L subclones as determined by densitometry after normalization with GADPH. Columns means of three individual experiments, bars SE. Statistics were calculated by comparing drug-treated cells with control cells; *p < 0.05; **p < 0.01