Figure 4.

MDM2 inhibits localization of REST on the p85 promoter, and inhibits p85 expression. ChIP analysis of p85 promoter in extracts from H1299 cells expressing MDM2 or its deletion mutants Del 1–120 and Del 491–155 using a REST antibody (a). ChIP using IgG control is shown in each case. Expression of p85 transcript by QPCR analysis (b) and protein by western blot analysis (c) in H1299 cells expressing MDM2 or its deletion mutants are shown. Migration of MDM2 or its deletion mutants, p85 and Erk2 are indicated using arrows