Figure 3.

HDACIs regulation of neutrophil apoptosis. (a) Apoptosis was assessed by flow cytometry using triple staining with Ly6G-APC antibody to detect neutrophil population and FITC-AnxAV/PI staining, to detect apoptosis on Ly6G⁺ cells. (b) Bone marrow cells from WT and AnxA1−/− mice were incubated for 6 h in vehicle, or treated with the reported concentrations of VPA, SB, TSA. Data are reported as increase of total apoptosis (AnxAV⁺) over vehicle-treated cells and represent the cumulative data of three independent experiments, each one with two mice. *P<0.05, one-way ANOVA WT versus AnxA1−/−, followed by Dunnett's correction