Figure 2.

2-DG induces the activation of Akt. (a) LNCaP were incubated with 20 mM 2-DG for the indicated times, and an immunoblot of PAkt Ser⁴⁷³ and Thr³⁰⁸ was performed. The graph represents the quantification of three independent experiments. (b) LNCaP were treated with 20 mM 2-DG for 8 h before the immunobloting of the indicated proteins. (c) LNCaP cells were transfected with siRNA against Akt1 and Akt2 for 48 h and treated with 2-DG for 48 h before the caspase 3 assay. The immunoblot represents the level of Akt before the caspase assay. The experiment is representative of three independent experiments, with each condition in triplicate. The differences were significant with P<0.05 (*)