Figure 4.

Overexpression of AICD restores the CPT-induced PML expression. (a) Western blot analysis revealed that the transient transfection of AICD cDNA into PS dKO MEFs upregulated CPT-induced PML expression compared with mock transfection. (b) Densitometric analysis of PML protein expression from four independent experiments. PML was normalized to α-tubulin. ^***P<0.001, ^###P<0.001. (c) Cotransfection with AICD and Fe65, which is known as AICD adaptor protein, into HEK293 cells significantly increased the transactivation of PML promoter compared with control or AICD-transfected groups. The values of three independent experiments were represented as mean±SEM. ^**P<0.01, ^***P<0.001, ^###P<0.001. (d) Transfection efficiency of PML, AICD and Fe65 was confirmed by western blot analysis. (e) APP siRNA into PS WT MEFs downregulated CPT-induced PML, p53 expression compared with control siRNA. (f) Densitometric analysis of PML protein from three independent experiments. PML was normalized to α-tubulin. ^**P<0.01, ^#P<0.05