Figure 2.

ASPP2 colocalises with and contributes to RAS activation at the cell membrane. (a) U2OS cells co-transfected with ASPP2 (red) and HRASV12 (green) show colocalisation of both at the cell membrane (yellow). (b) Immunofluorescence staining of HRAS in HKe3 ER:HRASV12 cells transfected with control or ASPP2 siRNA for 3 days, followed by treatment without (control) or with 100 nM 4-OHT for 1 h. Arrows indicate cell membrane. (c) Immunofluorescence staining of ASPP2 or HRAS in ASPP2^(+/+) or ASPP2^(Δ3/Δ3) MEFs, with or without HRASV12 expression, before or after treatment as indicated. Cells were treated with 1 μg/ml insulin for 15 min. Arrows indicate cell membrane