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. 2013 Feb 8;20(4):525–534. doi: 10.1038/cdd.2013.3

Figure 5.

Figure 5

RAS oncogene enhances the apoptotic function of p53 via ASPP1 and ASPP2. (a) ASPP1 and ASPP2 work with endogenous RAS to stimulate apoptosis. shRNA against HRAS or KRAS was co-transfected with ASPP1 or ASPP2 in U2OS or MCF7 cells. FACS analysis was used to determine apoptotic cells. (b) Western blots showing the expression levels of p53 and transfected ASPP1 and ASPP2. PCNA was used as a loading control. (c) Apoptotic activity is detected following oncogenic RAS activation. HKe3 ER:HRASV12 cells were treated with 4-OHT (100 nM) for the indicated time. Western blot showing expression levels of cleaved PARP and ASPP2. (d) p53 depletion reduces oncogenic RAS-induced apoptosis. HKe3 ER:HRASV12 cells transfected with control siRNA or siRNA against p53 for 3 days were treated with or without 4-OHT (100 nM) for another day. Western blots showing expression levels of cleaved PARP and p53