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. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: J Chromatogr A. 2012 Nov 19;1271(1):71–85. doi: 10.1016/j.chroma.2012.11.024

Fig. 13.

Fig. 13

Separation of carbobenzoyloxy (Z)-tripeptides differing in the terminal amino-acid residues. Experimental conditions: Apparatus and column: see Fig. 10 caption; solvent system: methyl tert.-butyl ether–1-butanol–acetonitrile–water (2:2:1:5, v/v), 16 mM TFA in the organic stationary phase (pH 2.00), 2.7 mM NH3 in the aqueous mobile phase (pH 10.35); sample: 5 Z-tripeptides indicated in the chromatogram, each 100 mg dissolved in 50 ml of solvent (25 ml each phase); flow rate: 3.3 ml/min; elution mode: head to tail; detection: 206 nm; revolution: 800 rpm (600 rpm for first 66 ml elution); stationary phase retention: 59.4%.