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. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: J Chromatogr A. 2012 Nov 19;1271(1):71–85. doi: 10.1016/j.chroma.2012.11.024

Fig. 14.

Fig. 14

Chromatograms of four isomeric pairs of dipeptides obtained by the standard HSCCC using ligand-free stationary phase (A) and an affinity ligand (DEHPA) in the stationary phase (B). Experimental conditions: Apparatus and column: see Fig. 10 caption; solvent system: methyl tert.-butyl ether–0.1 M KH2PO4 (1:1, v/v), (B) 10% DEHPA added to the organic stationary phase; elution: HCl at 0.035 M added stepwise to the mobile phase after 1 h and at 0.1 M after 2 h to shorten the separation time; flow rate: 3.3 ml/min; sample: 5 mg each of eight dipeptides indicated in the chromatogram; detection: 280 nm; revolution: 800 rpm.