Skip to main content
. Author manuscript; available in PMC: 2014 Jan 4.
Published in final edited form as: J Chromatogr A. 2012 Nov 19;1271(1):71–85. doi: 10.1016/j.chroma.2012.11.024

Fig. 21.

Fig. 21

Chiral separation of (±)-DNB-leucine by pH-zone-refining CCC using DPA as a chiral selector. Experimental conditions: Apparatus and column: see Fig. 10 caption; solvent system: methyl tert.-butyl ether–acetonitrile–water (4:1:5 v/v), TFA (40 mM) + DPA (40 mM) in the organic stationary phase and NH3 (20 mM) in the aqueous mobile phase; sample: (±)-DNB-leucine 2 g; flow rate: 1 ml/min; elution mode: head to tail; detection: 206 nm; revolution: 800 rpm. The associated analytical HSCCC analyses were carried out using the same column that was used for the standard CCC technique under the following experimental conditions: solvent system: hexane–ethyl acetate–methanol–10 mM HCl (8:2:5:5, v/v), organic stationary phase containing DPA (20 mM); flow rate; 1 ml/min; elution mode: head to tail; revolution: 800 rpm.