Fig. 2.

Co enhancement of the LO promoter activity stimulated by HIF-1 in treated RFL6 cells. Cells were cotransfected with the LO promoter-reporter construct (pLOProm 804) and different HIF-1 expression vectors as shown, as well as the internal control phRL-TK vector, then treated without or with 100µM Co for 24h. Luciferase activity in each treatment was normalized to the internal control and expressed as % of the control in which cells were cotransfected with the pcDNA3.1 basic vector without the HIF-1 cDNA insert. *p < 0.05 and ***p < 0.001 relative to control cells cotransfected with the pcDNA3.1 basic vector.