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. 2013 Jan 4;132(2):368–378. doi: 10.1093/toxsci/kfs345

FIG. 4.

FIG. 4.

AhR activation reduces FSK-induced Per1 through CRE but not through E-box elements. (A) c7 cells were seeded in a 12-well plate. After 24h, E-box decoy or mis-sense ODN was transfected into cells with Lipofectamine. After overnight incubation, cells were pretreated for 30min with 10µM of BNF or vehicle and then exposed to 10µM of FSK or vehicle for 1h. Per1 mRNA was examined by qPCR. n = 3, ****p < 0.0001 comparing FSK with all other groups by two-way ANOVA using Bonferroni’s post hoc comparison. (B) c7 and c12 cells were transfected with Per1-Luc or CRE-Luc using lipofectamine. The Per1-Luc served as a positive control. After overnight incubation, cells were treated with 100ng/ml of TPA + 10μM of FSK for 6h. Promoter activity was evaluated by luciferase assay. Firefly activity was normalized to Renilla luciferase. n = 3, *p < 0.05, ***p < 0.001, and ****p < 0.0001 comparing DMSO with FSK + TPA for both cell types and luciferase constructs with two-way ANOVA and Bonferroni’s post hoc comparison.