FIG. 5.

AhR activation reduces FSK-induced Per1 through the JNK pathway. (A) c7 cells were pretreated for 30min with vehicle or 10µM of BNF and then were treated with 10μM of FSK for 0, 10, 30, and 60min. *p < 0.05 compared with FSK group at individual different time points with Student’s t-test. (B) c12 cells were pretreated for 30min with vehicle or 10µM of BNF and then were treated with 10μM of FSK for 0 or 60min. (C) c7 cells were treated for 30min with vehicle or pJNK inhibitor 10μM of SP600125, then for another 30min with vehicle or 10µM BNF, and finally with vehicle or 10µM FSK for 1h. Protein was collected and immunoblot performed for pCREB and p-JNK (representative blot). (D) Densitometry for immunoblotting experiment described in C. (E) Cells were treated as in C; RNA was collected; and qPCR was performed to examine Per1. Immunoblot was performed to examine p-CREB and p-JNK. β-Actin is used as internal control for immunoblot and qPCR. n = 3–5, *p < 0.05 compared with FSK or SP + BNF + FSK by one-way ANOVA with Tukey’s post hoc comparison for C and D.