Table 1.
Proteins significantly changed by cocaine self-administration and extinction
| Accession number | Protein | 95% credible intervala | No. of peptides | Protein coverage | Mascot score |
|---|---|---|---|---|---|
| Cytoskeletal | |||||
| gi|31543764 | α-Spectrin 2 | 1.01–1.09 | 81 | 0.3989 | 4868 |
| gi|13591902 | Actinin, α1 | 1.03–1.21 | 13 | 0.1839 | 549 |
| gi|9506811 | Internexin neuronal intermediate filament protein, α | 1.13–1.33 | 10 | 0.2099 | 743 |
| gi|13928704 | Myosin, heavy polypeptide 10, non-muscle | 1.01–1.20 | 10 | 0.0855 | 134 |
| gi|13929098 | Neurofilament, light polypeptide | 1.03–1.24 | 8 | 0.1439 | 350 |
| gi|11560133 | Tubulin, α1A | 0.74–1.00 | 2 | 0.0599 | 831 |
| gi|109506033; gi|109505063 | Similar to actinin α2; similar to actinin α2 | 1.03–1.34 | 4 | NaN | 451 |
| Synaptic | |||||
| gi|27465607 | Discs large (Drosophila) homolog-associated protein 3 | 1.01–1.32 | 4 | 0.0583 | 245 |
| gi|11560135 | Brain abundant, membrane attached signal protein 1 | 1.13–1.27 | 19 | 0.8227 | 1947 |
| gi|164663821 | Synaptic Ras GTPase activating protein 1 homolog isoform 1 | 1.00–1.16 | 16 | NaN | 587 |
| gi|8393415 | Growth associated protein 43 | 1.18–1.35 | 15 | 0.7168 | 1111 |
| gi|158749559 | Bassoon protein | 1.10–1.24 | 30 | 0.1353 | 1237 |
| gi|6981602 | Syntaxin binding protein 1 | 0.82–0.97 | 9 | 0.2239 | 411 |
| gi|11067399 | SH3/ankyrin domain gene 3 | 1.03–1.23 | 9 | 0.0885 | 609 |
| gi|13591936 | Drebrin 1 | 1.04–1.35 | 4 | 0.0665 | 186 |
| gi|158303339 | Synaptopodin | 1.03–1.31 | 5 | 0.1301 | 184 |
| gi|19424156 | A-kinase anchor protein 5 | 1.00–1.26 | 5 | 0.0966 | 357 |
| Cell signaling | |||||
| gi|6978593 | Calcium/calmodulin-dependent protein kinase II, α | 1.03–1.18 | 14 | 0.3054 | 2604 |
| gi|108796657 | Calcium/calmodulin-dependent protein kinase II β subunit | 1.05–1.25 | 7 | 0.2288 | 1295 |
| gi|57977323 | 2′,3′-cyclic nucleotide 3′ phosphodiesterase | 0.83–0.94 | 21 | 0.5357 | 883 |
| Channels/receptors/transporters | |||||
| gi|13786200 | Voltage-dependent anion channel 1 | 1.00–1.17 | 10 | 0.4664 | 1024 |
| gi|32189355 | Solute carrier family 25, member 4 | 0.78–0.94 | 7 | 0.2215 | 501 |
| gi|31542335 | Calcium channel, voltage-dependent, a2/d subunit 1 | 1.06–1.31 | 6 | 0.0862 | 208 |
| gi|32189350 | Solute carrier family 25, member 5 | 0.77–0.96 | 5 | 0.2081 | 332 |
| gi|9507115 | Glial high affinity glutamate transporter, member 3 (GLAST) | 0.74–0.99 | 2 | 0.0516 | 109 |
| Metabolism | |||||
| gi|16758362 | Cytochrome c oxidase subunit Vb | 1.04–1.24 | 9 | 0.6279 | 245 |
| gi|157819953; gi|109493236; gi|109493234 | ATPase, H transporting, lysosomal V1 subunit A | 1.02–1.22 | 9 | NaN | 887 |
| gi|148747253 | ATPase, Na+/K+ transporting, β1 polypeptide | 0.77–0.92 | 8 | 0.2664 | 397 |
| gi|109467443; gi|109465447; gi|109458527 | Similar to cytochrome c oxidase, subunit VIb polypeptide 1 | 0.84–1.00 | 8 | NaN | 566 |
| gi|8393180 | Cytochrome c oxidase subunit IV isoform 1 | 1.05–1.26 | 7 | 0.3728 | 346 |
| gi|170295834; gi|109487466 | NADH dehydrogenase (ubiquinone) 1α subcomplex 10 | 0.81–0.98 | 7 | NaN | 581 |
| gi|6978545 | ATPase, Na+/K+ transporting, α2 polypeptide | 0.72–0.89 | 6 | 0.0863 | 1466 |
| gi|109474252 | Similar to NADH dehydrogenase (ubiquinone) 1α subcomplex, 9 | 0.82–0.97 | 11 | 0.4138 | 475 |
| gi|6978547 | Na+/K+-ATPase a3 subunit | 0.76–0.90 | 11 | 0.1441 | 2201 |
| gi|24233541 | Cytochrome c oxidase, subunit Va | 1.07–1.23 | 11 | 0.6027 | 733 |
| gi|34866853; gi|109482351 | Similar to cytochrome c-1; similar to cytochrome c-1 | 1.00–1.25 | 5 | NaN | 324 |
| gi|114145517 | NADH dehydrogenase (ubiquinone) 1α subcomplex, 8 | 0.76–0.99 | 3 | 0.1693 | 47 |
| gi|72086149 | NADH dehydrogenase (ubiquinone) Fe-S protein 5b | 0.69–0.95 | 2 | 0.2547 | 132 |
| Cell adhesion | |||||
| gi|13591880 | Proteolipid protein (myelin) 1 | 0.70–0.89 | 4 | 0.1408 | 98 |
| gi|16923964 | Contactin 1 | 1.04–1.21 | 14 | 0.1459 | 805 |
| gi|70166255 | Myelin basic protein isoform 2 | 1.01–1.16 | 12 | 0.5799 | 769 |
| Unknown | |||||
| gi|62079059 | BM88 antigen | 1.02–1.33 | 3 | 0.2550 | 231 |
Significantly changed proteins are indicated within groups by function. Upregulated proteins are indicated in regular type, and downregulated proteins are indicated in bold type. The number of peptides identified by MS/MS for each protein are indicated, as well as average percentage change among identified proteins and fraction of the entire protein sequence covered by identified peptides. NaN indicated Not a Number, meaning that the protein was not found in the protein sequence file used to construct the report and so the sequence coverage could not be computed, in a case in which the protein database searched by Mascot is different from that used to produce the report. Assessment of treatment-dependent changes in protein expression using 95% credible intervals as described by Hill et al. (2008) using computational methods and software described by Schwacke et al. (2009). Mascot scores were calculated using the Mascot algorithm (Matrixscience).
aWhen the credible interval is 1, the protein is significantly (p < 0.05) different between the cocaine and yoked saline groups.