Figure 5. Different PROPPINs bind similarly to PI(3)P, PI(3,5)P2, and PI(5)P.
Pulldown assays with 15% phosphatidylinositol phosphate liposomes show PROPPIN binding to phosphatidylinositol 3-phosphate, 5-phosphate and 3,5-bisphosphate. The final concentration of the proteins in the pulldown experiment was 1.5 µM, identical to that used for assays shown in Figures 2 and 3.