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. Author manuscript; available in PMC: 2013 Aug 10.
Published in final edited form as: Mol Cell. 2012 Jun 14;47(3):339–348. doi: 10.1016/j.molcel.2012.05.027

Figure 7. Sites 1 and 2 and the blade 6 loop aromatic residues are important for autophagy.

Figure 7

  1. GFP-Atg8 processing monitored by western blot against GFP. The full length GFP-Atg8 and GFP bands are labeled. The actin loading control is shown below.
  2. Representative microscopy images for GFP-Atg8 shown on the left and transmitted light images shown on the right.
  3. The percentage of cells displaying GFP-Atg8 puncta with a total of 100 cells counted.
  4. Pho8Δ60 alkaline phosphatase specific activity normalized to starved cells expressing Atg18-myc. Data for non-starved cells are shown in white and starved cells are shown in gray. Error bars in (C) and (D) represent the standard deviation of triplicate experiments.