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a) Hematoxylin and eosin (H & E) stain of an untreated rat cerebellum region with outlined (yellow) region of where the hydrogel was placed on the serial digested tissue (scale bar 250 µm) and b) the H & E stain of the serial sectioned, previously imaged (Figure 3) and hydrogel digested rat cerebellum (scale bar 50 µm).
