Table 1.
High molecular weight (> 100 kDa) proteins identified with on-tissue hydrogel-mediated protein digestion and extraction on rat brain cerebrum.
| Protein Name | UniProt Accession Number |
Molecular Weight (kDa) |
# Unique Peptides |
# Assigned Spectra |
|---|---|---|---|---|
| Hexokinase type 1 | P05708 | 102 | 4 | 6 |
| α actinin 1 | Q6GMN8 | 103 | 5 | 10 |
| Puromycin-sensitive aminopeptidase | P55786* | 103 | 4 | 6 |
| Type II brain 4.1 minor isoform | Q9JMB2 | 107 | 3 | 5 |
| Na+/K+ ATPase α3 subunit | P06687 | 112 | 20 | 67 |
| Na+/K+ transporting ATPase α2 chain precursor | D3ZSA3 | 112 | 3 | 6 |
| Na+/K+ transporting ATPase α1 chain precursor | P06685 | 113 | 10 | 17 |
| Contactin-1 precursor | Q63198 | 113 | 6 | 9 |
| Neurofilament heavy polypeptide | F1LRZ7 | 114 | 8 | 11 |
| Ubiquitin-activating enzyme E1 | Q5U300 | 118 | 3 | 4 |
| Electroneutral K+/Cl− cotransporter 2 | Q63633 | 124 | 2 | 2 |
| Plasma membrane Ca2+ ATPase isoform 2 | P11506 | 137 | 6 | 9 |
| Neurofascin | P97685 | 138 | 2 | 2 |
| Plasma membrane Ca2+ transporting ATPase 1 | P11505 | 139 | 3 | 4 |
| Ras GTPase-activating protein SynGAP | Q9QUH6 | 143 | 3 | 3 |
| Tenascin-R precursor (Restrictin) | Q92752* | 150 | 2 | 3 |
| Clathrin heavy chain 1 | P11442 | 191 | 9 | 13 |
| Microtubule-associated protein 2 | P15146 | 199 | 21 | 46 |
| Microtubule-associated protein 1B | P15205 | 270 | 8 | 13 |
| Spectrin β chain, brain 2 | Q9QWN8 | 271 | 5 | 7 |
| Non-erythroid spectrin β | Q6XD99 | 274 | 29 | 47 |
| Spectrin α chain, brain | P16086 | 285 | 60 | 109 |
| Microtubule-associated protein 1A | G3V7U2 | 300 | 15 | 27 |
| Protein bassoon | O88778 | 418 | 2 | 4 |
| Ankyrin-2 | Q01484* | 437 | 5 | 5 |
| Dynein heavy chain, cytosolic | P38650 | 532 | 8 | 9 |
*
Predicted protein based on sequence homology from Homo sapiens