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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Biochem J. 2009 Feb 1;417(3):695–703. doi: 10.1042/BJ20081706

Figure 2. Profile of transcription factor binding at the ASNS proximal promoter during UPR activation.

Figure 2

ChIP analysis was performed on HepG2 cells treated for 0–12 h with control medium (MEM) or MEM containing Tg (MEM+Tg) to induce ER stress. Antibodies against RNA Pol II, ATF4, ATF3 and C/EBPβ were used for the immunoprecipitation step and primers specific for the ASNS promoter region were used for qRT–PCR amplification. Each PCR reaction was run in duplicate and the data were obtained from at least three independent experiments. The data are shown as fold-change relative to the MEM control and represent the means ± S.E.M. The dashed line in the top panel represents the ASNS transcription activity for the Tg-treated cells in Figure 1.