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. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Biochem J. 2009 Feb 1;417(3):695–703. doi: 10.1042/BJ20081706

Figure 4. ASNS transcriptional induction during ER stress does not involve enhanced recruitment of mediator subunits.

Figure 4

HepG2 cells incubated in MEM control medium only (white bars) or MEM+Tg (black bars) for 1 h and 8 h were used to perform ChIP analysis using antibodies specific for RNA Pol II (A), the MED1, MED23 or CDK8 Mediator subunits (B and D), or a non-specific rabbit IgG (ns/IgG), as indicated. During the final qRT–PCR step, primers specific for the ASNS promoter region or BiP/GRP78 were used (A, B and D). The transcriptional activity of BiP/GRP78 (C) was measured using primers that span the exon 2 – intron 2 junction. The qRT–PCR reactions were performed in duplicate for each sample. The ChIP data were collected from at least three independent experiments and the values represent the means ± S.E.M; *P <0.05.