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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Transplantation. 2012 Mar 27;93(6):580–588. doi: 10.1097/TP.0b013e318244dd67

Figure 5. Alloantigen induced Tregs prolong islet allograft survival in a fully H-2 mismatched, non-lymphopenic immunocompetent mouse islet cell transplantation model and allograft protection is alloantigen specific.

Figure 5

(A) A total of 500 B6 islets were transplanted under the renal capsule of diabetic BALB/c mice without Tregs, with 5×105 alloantigen induced Tregs (alloTregs), or 5×105 freshly isolated nTregs on Day 0. The alloTregs were generated in MLR using BALB/c CD4+ T cells as responders and irradiated B6 DCs as stimulators and the MLRs were supplemented with IL-2, and TGF-β1. Mean (±SE) survival times for B6 islet grafts transplanted without Tregs (blue, N=18 recipients), with alloTregs (green, N=9 recipients) or nTregs (red, N=8 recipients) are shown. Allograft rejection was defined as two consecutive blood glucose measurements exceeding 250mg/dL. (B) A total of 500 CBA islets were transplanted under the renal capsule of diabetic BALB/c mice without Tregs (blue, N=7 recipients) or along with 5×105 alloTregs (green, N=5 recipients) on Day 0. The alloTregs were generated in MLR using BALB/c CD4+ T cells as responders and irradiated B6 DCs as stimulators and the MLRs were supplemented with IL-2, and TGF-β1. P values calculated using Kaplan-Meier survival analysis and the log rank test.