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Schematic illustration of (RGD-PEG) modification patterns and HK siRNA nanoplex formation. Each of four N-terminal branches was conjugated to a single (RGD-PEG). A two-step mixing was utilized to prepare modified nanoplexes: (RGD-PEG)4H3K(+H)4b was first added to a helper peptide, H2K4b. The resulting mixture was then mixed with siRNA at room temperature for 40 min to form HK siRNA nanoplexes.
