Figure 7.
MDA-MB-435 tumor growth and Raf-1 mRNA inhibition. (A) MDA-MB-435 tumor-bearing mice were separated into four groups when tumor size was about 25 mm3: untreated, H3K(+H)4b/siLuc control, H3K(+H)4b/Raf-1 siRNA, and RP-HK/Raf-1 siRNA. Treatment was given three times a week for 2 weeks (arrows). Data for each time point represent the mean ± SD of tumor volume of five determinations for each treatment group. **, P < 0.01. (B) Raf-1 and β-actin mRNA expression was determined by RT-PCR analysis of RNA isolated from tumor tissue at day 22. Lanes 1–3 represents untreated, H3K(+H)4b/Raf-1 siRNA and RP-HK/Raf-1 siRNA respectively.