Figure 1. Adenovirus maturation and AFM imaging.

(a) Sketch representing the energetics of viral disassembly. (b) Schematic representation of ts1 (immature) and WT (mature) adenovirus structure. (c) Cartoon depicting the Jumping Mode imaging force cycles (red), as well as the z-piezo actuation (black) along a single scan-line. (d) AFM topography of adenovirus in liquids, presenting a view along the 3-fold symmetry axis. (e) High pass filtered version of the central triangular facet of (d) revealing the towers of the triangular hexons cued by black dots. The red topographical profile inset is taken along the white dashed line of (e). An inset showing a hexon trimer as imaged by cryo-electron microscopy³⁶ is shown with black dots indicating each tower for comparison.