Skip to main content
. Author manuscript; available in PMC: 2013 Mar 13.
Published in final edited form as: Mol Cell. 2012 Sep 27;48(3):445–458. doi: 10.1016/j.molcel.2012.08.022

Figure 1. Identification of Notch1-Associated Nuclear Factors.

Figure 1

(A) Purification of NOTCH1 intracellular domain (ICN1) and its interacting partners. Nuclear extracts (NEs) prepared from FLAG-HA epitope-tagged ICN1 (F/H-ICN1)-expressing SupT1 cells or mock-transduced SupT1 cells were subjected to sequential immunoprecipitation using anti-FLAG and anti-HA beads. Proteins were resolved by SDS-PAGE and visualized by silver staining.

(B) Interaction network of ICN1-associated proteins identified by Mass Spectrometry (MS). The false positive interactors were excluded by removing all proteins that were also detected in the control purification. See also Figure S1 and Tables S1, S2, and S3.