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. 2012 Jan;26(1):17–23. doi: 10.1016/S1674-8301(12)60003-7

Fig. 3. Expression and purification of sRpfE on 15% SDS-PAGE gel and Western blot analysis using anti-His6 antibody.

Fig. 3

Lane M: molecular weight marker; Lanes 1-3: elution with native elution buffer; Lane 4: Western blot analysis of lysate of M. vaccae (pDE22-rpf) using His antibody; Lane 5: Western blotting analysis of lysate of M. vaccae (pDE22) using His antibody; Lane 6: purified sRpfE.