. Author manuscript; available in PMC: 2013 Oct 24.

Published in final edited form as: J Am Chem Soc. 2012 Oct 10;134(42):17428–17431. doi: 10.1021/ja308203h

Table 1.

Fluorescence properties of azido- and triazolylfuoresceins in pH 7.4 PBS. “1–12” denotes the triazolylfluorescein product from reaction of 1 and 12. Other triazolylfluoresceins are named analogously

Compound	λ_ex	λ_em	Φ₅₀₀	Increase
fluorescein	490	510	--	--
1	497	513	0.024	--
1–12	495	518	0.70	29x
1-DIFO	499	517	0.81	34x
1-DIMAC	499	517	0.72	30x

2	501	521	0.0005	--
	6
2–12	506	517	0.0018	3.2x
2-DIFO	507	522	0.0018	3.2x
2-DIMAC	507	521	0.0007	1.4x
	6

3	496	516	0.057	--
3–12	499	520	0.72	13x
3-DIFO	499	519	0.49	8.6x
3-DIMAC	499	518	0.72	13x
4	497	518	0.0052	--

4–12	499	519	0.13	25x
4-DIFO	497	520	0.019	3.7x
4-DIMAC	497	516	0.020	3.8x

N₃-fluor	492	511	0.75	--
N₃-fluor-12	494	516	0.59	0.79x