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. 2012 Dec 7;14(2):191–198. doi: 10.1038/embor.2012.196

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Copyright © 2013, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial No Derivative Works 3.0 Unported License, which permits distribution and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission.

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Recruitment of Cdc45 and Pol2 to replication origins. (A) DNA content analysis of AKY543 strain at indicated time-points after release from G1 arrest. (B–H) Recruitment of Cdc45 and Pol2 proteins to various replication origins throughout the S phase. The relative binding of the factors to the origins at indicated time-points are shown, 1 was defined as the average value of unbound samples. Error bars indicate s.d. of three experiments. ARS, autonomously replicating sequence.