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. 2012 Dec 7;14(2):191–198. doi: 10.1038/embor.2012.196

Figure 4.

Figure 4

Activation of ARS607 in ectopic loci. (A) Schematic representation of ARS305, ARS737, ARS607 and their mutants. Deletions of ARS607 and the sites of point mutations of all origins are shown. Sequences corresponding to the Fkh1/2 consensus sequence RTAAAYA [33] are indicated as grey boxes, ACS is shown in bold, B3 and B4 boxes in ARS607 [7] and ARS305 [3] are underlined. (B–I) Relative binding of Cdc45 to indicated wild-type and mutated ARS sequences in the VPS13 locus. The binding of Cdc45 to the origin-free VPS13 is shown for reference in all panels. (J) Activation of ARS607 in different genomic loci. Relative binding of the Cdc45 protein to the ARS607 sequence inserted into VPS13, DPB11, HXK1 or CLD1 loci was determined. The binding of Cdc45 to genuine ARS607 and origin-free VPS13, HXK1 and CLD1 loci is shown for reference. Error bars indicate s.d. of three experiments. ARS, autonomously replicating sequence; mut, mutation.